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Fig. 6. Robust optic nerve regeneration and target innervation in Bcl-2tgGFAP^–/–Vim^–/– mice after P5. (A-D) Photomicrograph montages of adjacent longitudinal optic nerve sections on day 4 after optic nerve crush in P5 GFAP^–/–Vim^–/– (G/V) (A,B) and Bcl-2tgGFAP^–/–Vim^–/– (Bcl-2tg/G/V) (C,D) mice. The asterisk indicates the crush site. Bar, 100 µm. (E-G) Higher-power views of axon and growth cone morphology (40x) revealed by anti-NF staining. Bars, 40 µm (E); 20 µm (F,G); 5 µm (H-K). Epifluorescence photomicrographs of coronal brain sections from GFAP^–/–Vim^–/– (H,J) and Bcl-2tgGFAP^–/–Vim^–/– (I,K) mice subjected to optic nerve crush on P14 and examined 4 days later. Note the positive labeling in the ipsilateral optic chiasm (K), dLGN and vLGN, and the optic tract (arrowheads) (I) of Bcl-2tgGFAP^–/–Vim^–/– brain sections. Dotted lines outline the dLGN. Bar, 200 µm.

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