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Fig. 1. Subcloning NMuMG cells. (A) Parental NMuMG cells (a-c), clone NMuMG/E9 (d,e) and clone NMuMG/E11 (f,g) were stained for N-cadherin (a,d,f) and E-cadherin (b,e,g). (c) A merged picture of a,b. (a-c) Photographs were taken using a 10x objective; scale bar, 50 µm. (d-g) Photographs were taken using a 40x dry objective; scale bar, 10 µm. (B) TNE extracts of NMuMG/E9 (lane 1) and NMuMG/E11 (lane 2) were analysed for N-cadherin (top) and E-cadherin (bottom) by immunoblots. (C) Both clones underwent morphological changes (elongation of cell shape, b,d) in response to TGF-ß1 [Tß1; 5 ng ml–1 for 1 day (d)]. Photographs were taken of living cells using a 10x objective; scale bar, 50 µm.





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