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Fig. 10. N-Cadherin overexpression promoted motility in MCF10A cells. (A) Phase-contrast micrographs of living cells (a,b; 10x objective; scale bar, 50 µm) and N-cadherin immunostaining micrographs (c,d; 40x objective; scale bar, 10 µm) of parental MCF10A cells (a,c) or MCF10A cells overexpressing 2x-Myc-tagged human N-cadherin (MCF10A/Ncad; b,d). (B) Phase-contrast micrographs of living cultures of parental MCF10A cells (a-c), MCF10A/ctr KD cells (d-f) or MCF10A/Ncad cells (g-i) in a wound-healing assay performed in the absence of TGF-ß1. Photographs were taken immediately after incision (0 hour; a,d,g) and at 13 hours (b,e,h) or 27 hours (c,f,i) after incision using a 10x objective; scale bar, 50 µm. Some of these panels are the same as those shown in Fig. 9A, for easier comparison to MCF10A/Ncad cells. (C) Wound-healing assays were quantified as described in Fig. 9. MCF10A/Ncad cells were significantly more motile than either control cell line at 13 hours and at 27 hours. (D) Transwell motility assay comparing parental MCF10A cells, MCF10A/ctr KD cells and MCF10A/Ncad cells in the absence of TGF-ß1. Data are presented as the average number of migrating cells in nine random fields of view in three independent experiments.

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