spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. TGF-ß1-mediated EMT in NMuMG/E9 cells. (A) NMuMG/E9 cells were stained for E-cadherin (a,b), ZO-1 (c,d), paxillin (e,f) and F-actin (phalloidin; g,h) in the absence of TGF-ß1 (a,c,e,g) or in the presence of TGF-ß1 (5 ng ml–1 for 1 day; b,d,f,h). In the presence of TGF-ß1, the signals for E-cadherin and ZO-1 became weak and punctate, and focal adhesions (f) and stress fibers (h) were induced. Photographs were taken using a 63x oil objective; scale bar, 10 µm. (B) NMuMG/E9 cells were treated with TGF-ß1 and cultured without passage for the indicated times. TNE extracts were immunoblotted for N-cadherin, E-cadherin, ß-catenin and GAPDH at the indicated times from t=0 hour (h) to t=9 days (d). Immunoblots were quantified and normalized to GAPDH as shown on the graph. ZO-1 (an epithelium marker) and fibronectin (a mesenchyme marker) were examined in the same time course.





Right arrow Return to article