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Fig. 4. Snail and SIP1 were induced during TGF-ß1-mediated EMT in NMuMG/E9 cells. (A) RT-PCR using total RNA extracted from untreated (lane 1) and TGF-ß1-treated (5 ng ml1 for 2 days) NMuMG/E9 cells (lane 2) was done for snail, SIP1, slug, E12/E47 and ß-actin (as a control). The identity of the slower migrating band in the E12/E47 lane is not known. (B) RT-PCR using total RNA extracted from NMuMG/E9 cells over the same time course as Fig. 2B. E-Cadherin, snail, SIP1 and GAPDH were analysed. (C) The snail and SIP1 mRNA levels were analysed by quantitative real-time RT-PCR using the same total RNA as in B.