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Fig. 7. E-Cadherin and N-cadherin expression in MCF10A cells was dependent on cell confluence. (A) Phase-contrast micrographs of MCF10A cells in sparse culture (a) or confluent culture (b). Photographs were taken of living cells using a 10x objective; scale bar, 50 µm. (B) MCF10A cells in sparse culture (a,c) or confluent culture (b,d) were stained for E-cadherin (a,b) or N-cadherin (c,d). Photographs were taken using a 40x dry objective; scale bar, 10 µm. (C) TNE extracts of MCF10A cells in increasing confluence were immunoblotted for N-cadherin (top), E-cadherin (middle) and ß-catenin (bottom).

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