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Fig. 2. Generation of stable IMCD cell populations expressing CD98 or CD98/CD69 chimeras. IMCD cells were transfected with the constructs illustrated. Transfected cell populations were sorted by flow cytometry utilizing antibodies to either the human CD98 or the human CD69 extracellular domain. The expression levels of the cell populations (solid lines) are shown relative to IMCD cells transfected with pcDNA3 vector only (dotted lines). C, cytoplasmic domain; T, transmembrane domain; E, extracellular domain.





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