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Fig. 7. The five N-terminal amino acids of the transmembrane domain of CD98 are required for CD98 association with ß1 integrin. (A) Equal amount of cell lysates (500 µg) from cell populations transfected with either control vector or the CD98 constructs indicated were immunoprecipitated with an anti-human CD98 (upper panel) or an anti-mouse ß1 integrin (third panel). The immunoprecipitates were separated by SDS-PAGE as indicated in Fig. 4 and membranes were incubated with an anti-mouse integrin ß1 (upper panel) or an anti-human CD98 antibody (third panel). Equal amounts of cell lysate were immunoblotted with an anti-human CD98 (second panel) or an anti-mouse integrin ß1 antibody (fourth panel) to demonstrate equal amounts of CD98 and ß1 integrin in the different cell populations.





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