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Fig. 8. Chagasin overexpression partially protects T. cruzi epimastigotes against the toxicity of the synthetic CP inhibitor N-Pip-F-hF-VSPh. Epimastigotes were inoculated at 10⁶ ml^–1 in LIT medium containing 10% FCS and 0.5% DMSO in the presence of 2 µM, 4 µM, 6 µM or 8 µM N-Pip-F-hF-VSPh, and cultivated for 5 days at 28°C. The controls were cultivated solely in the presence of the DMSO diluent. (A) WT. (B) MOCK. (C) pCHAG. The experiments were performed in triplicate and are reported as mean values with standard deviations (SD). The graphs are representative of two independent experiments. The analyses of variance were performed using two-way ANOVA and the Bonferroni post-test at a significance level of 5%. The single asterisks show the scores that are statistically significant at P<0.05 and the triple asterisks show the scores that are statistically significant at P<0.01. In the WT and MOCK individual graphs, the variation in the growth rate in the presence of all drug concentrations were equally significant in relation to growth in the presence of DMSO. The variances in the growth rates of pCHAG were significant in relation to the control and among the various drug concentrations. The variance in the growth rates of pCHAG in the presence of 6 µM and 8 µM N-Pip-F-hF-VSPh was extremely significant (P<0.0001) in relation to WT and MOCK.

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