JCS01675 Supplementary Material

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• Supplemental Figure -

  Fig. S1. Colocalisation of co-transfected TRPV channel subunits was analyzed by confocal laser-scanning microscopy. (A and B) Upper panels, subcellular localisation of the indicated coexpressed CFP- and YFP-tagged TRPV subunits. Lower panels, pixel-based image analysis of the CFP and YFP fluorescence intensities. Lower left, scatter histogram of pixel intensities in the CFP and YFP channels. Lower right, pixels that are grouped in the indicated areas of the intensity scatter histogram are depicted selectively. The correlation coefficient r² represents the square of the Pearson correlation coefficient:

  r = S (Ch1_i - Ch1_av) ´ (Ch2_i - Ch2_av) / [S (Ch1_i - Ch1_av)² ´ S (Ch2_i - Ch2_av)²]^1/2,

  where Ch1 and Ch2 are the fluorescence intensities determined for each pixel in channel 1 (YFP channel) and channel 2 (CFP channel).