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Fig. 5. Cytosolic termini are required for TRPV1 assembly and function. Schematic representation of N-terminal (A) and C-terminal (B) TRPV1 deletion mutants showing the ankyrin repeats (indicated as A) and the six transmembrane domains (1-6). WT represents full-length rat TRPV1. The numbers on the left indicate deleted amino acids. The table summarizes the data on the intracellular location of C-terminally YFP-tagged deletion mutants imaged by confocal laser-scanning microscopy, of the capsaicin-induced maximal increases in the intracellular Ca²⁺ concentrations ([Ca²⁺]_i), and of FRET efficiencies between mutants and full-length TRPV1 (A) or of FRET efficiencies in competition experiments with CFP- and YFP-tagged TRPV1 and non-tagged deletion mutants (B). [YFP]_r and [CFP]_r represent averaged relative fluorescence intensities of YFP-tagged TRPV1 or the N-terminal TRPV1 deletion mutants and TRPV1-CFP. Data are the mean±s.e. of four to eleven independent FRET experiments. ER, endoplasmic reticulum; PM, plasma membrane.

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