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Fig. 7. Interaction between cytosolic TRPV1 and TRPV4 termini. (A,B) Cytosolic N- and C-termini (NT, CT) of TRPV1 (A) or TRPV4 (B) tagged with either CFP or YFP were coexpressed in HEK293 cells as indicated and subjected to quantitative FRET analysis. Bars in panels A and B represent FRET efficiencies (means±s.e.) among TRPV termini (grey bars), TRPV termini and full-length TRPV channels (black bars) and, as a control, between TRPV termini and soluble CFP (white bars). Data shown are representative of at least three independent experiments. The average relative fluorescence intensities ([YFP]r and [CFP]r) indicate the relative expression levels of the respective TRPV constructs. (Insets) Confocal images showing typical localization of the YFP-tagged TRPV termini in HEK293 cells (bars, 10 µm).





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