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Fig. 8. Functional rescue of truncated TRPV1 or TRPV4 subunits by fusion with N-termini of related TRPV subunits. (A-C) Plasmids encoding YFP-tagged TRPV chimeras were transfected in HEK293 cells. In the presence of Mn2+ (250 µM), the cells were stimulated by adding 10 µM capsaicin (caps) or 5 µM 4{alpha}-phorbol 12,13-didecanoate (PDD) to the bath solution as indicated. Grey lines depict the time course of the total fura-2 fluorescence in single cells whereas the black lines represent the calculated means. The data are representative for three to four independent transfection experiments showing similar results. Insets show confocal micrographs of living HEK293 cells expressing the respective YFP-tagged TRPV chimera. Bar, 10 µm.





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