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Fig. 2. Skeletal analysis of {alpha}10-deficient mice. (A) Staining with Alcian Blue and Alizarin Red shows no obvious skeletal defect of mutant (m) mice compared to wild-type (wt) mice at the newborn stage. (B) Skeletal staining of hindlimbs of newborn (NB), 8-week-old (8w) and 12-week-old (12w) animals. (C) X-ray analysis of 1-year-old mice indicates a slight, time progressive shortening of the long bones. The insets in C show magnified images of the wild-type and mutant femur (arrows). (D) Diagram showing the relative length of the tibia and femur of {alpha}10 integrin-null mice compared to the wild type at various ages. Bars represent mean±s.d. A significant difference in bone length was measured in mutants compared to that in control mice *P<0.05, n=5 animals per genotype.





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