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Fig. 2. Translocation of Hic-5 from focal adhesions to actin stress fibers during uni-axial cyclic stretch. The Tet-Off cell lines of Hic-5 (A) and paxillin (B) were incubated without Dox for 24 hours, subjected to uni-axial cyclic stretching at 1 Hz for 60 minutes (+) or not (–) and stained with the antibody 12CA5 against HA-tag of Hic-5 (A, top) and paxillin (B, top). The cells were also stained with FITC-conjugated phalloidin (middle) and merged images of phalloidin and HA tag of Hic-5 or paxillin are shown on the right. SVS30 SM cells were subjected to uni-axial cyclic stretching at 1 Hz for 60 minutes (+) or not (–), and stained with the antibody against Hic-5 and with FITC-conjugated phalloidin (C) and with the antibody against paxillin and FITC-conjugated phalloidin (D). The arrow indicates the direction of stretching. Bars, 10 µm.





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