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Fig. 3. Intracellular distribution of Hic-5-binding proteins during uni-axial cyclic stretch. Mouse embryo fibroblast cells subjected to the uni-axial cyclic stretch for 60 minutes (+) or not (–), as in Fig. 2, were stained with antibodies against endogenous vinculin (A, top) and GIT1 (D, top). (B,C) The expression vector encoding myc-tagged FAK (B) or HA-tagged PTP-PEST (C) was introduced into mouse embryo fibroblast cells and, at 24 hours after transfection, the cells were subjected to cyclic stretching as above and stained with antibodies against tags [9E10 (B) and 12CA5 (C)]. The distribution of actin stress fibers was visualized by FITC-conjugated phalloidin labeling (A-D, middle). The arrow indicates the direction of stretching. Bars, 10 µm.
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