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Fig. 2. Heterochromatic association in CNS nuclei from developmentally arrested larvae. (A) The effect of feeding larvae erg2 ${Delta}$ yeast. Larvae were reared on wild-type yeast (top) or erg2 ${Delta}$ yeast (bottom). Irrespective of the yeast fed, the larvae enter the mid-third instar larval stage and appear to undergo normal development until 115 hours AED. At 135 hours AED the larvae fed erg2 ${Delta}$ yeast remain stalled in the third instar stage and eventually die after a few more days. By this time, larvae fed wild-type yeast have pupated (they usually pupate at 118-125 hours AED) and are ready to eclose 216 hours AED. (B) Box plots (as in Fig. 1) show data from FISH on CNS from bw⁺ and bw^D larvae. Larvae fed erg2 ${Delta}$ yeast were dissected at 110-115 hours AED (day 1) or 130-135 hours AED (day 2). Larvae fed wild-type (WT) yeast were dissected at 110-115 hours AED (day 1). P values (Mann-Whitney) are shown above brackets for the respective sets. (C) Histograms showing the distribution of the distances between 59E and 2Rh in bw^D larvae reared on WT yeast on day 1 and erg2 ${Delta}$ yeast on day 2.

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