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Fig. 5. Western blot analysis indicates that IP3Rs are resistant to detergent extraction. Pancreatic acini were homogenized and used to prepare a Triton X-100-soluble supernatant (S) and a Triton X-100-insoluble pellet (P). Proteins contained in the supernatant and pellet fractions were separated by SDS-PAGE and probed with antibodies recognising (A) actin, (B) myosin IIa and (C) IP3R3.





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