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Fig. 6. Latrunculin B inhibits Ca²⁺-dependent Cl^– current spikes in single whole-cell patch-clamped acinar cells. Cells were held under voltage clamp at a membrane potential of –30 mV. Trains of current spikes (downward deflections in the current, i.e. outward movement of Cl^–) were induced when 10 µM (2,4,5)IP₃ was present in the whole-cell patch pipette (A). Bath application of 50-90 µM latrunculin B led to a dose-dependent cessation of spiking (Ba and C). Application of 100 µM ACh at the end of the recording (Ba) showed the cells were still capable of mobilizing a response even after (2,4,5)IP₃-induced spikes were abolished. The ACh-induced response in the presence of latrunculin B (280±43 pA, mean±s.e.m.) was not different from control responses in the absence of latrunculin B (Bb) (397±57 pA, mean±s.e.m., Student's t-test P=0.79). DMSO, the drug vehicle, at the maximal concentration used in these experiments had no apparent effect on (2,4,5)IP₃-induced current spikes (D). The horizontal line on the left of the current records is the zero current line. The current-voltage relationship of currents induced by an intracellular solution containing 600 nM Ca²⁺ was not affected by treatment with latrunculin B (E).

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