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Fig. 3. Localization of GFP-tagged CPEB1 in HeLa cells. (A,B) Localization of CPEB1 after transient transfection. HeLa cells were transiently transfected with an expression vector for GFP-tagged human CPEB1-{Delta}5-long. After 24 hours, cells were fixed, stained with DAPI and observed by fluorescence microscopy. CPEB fluorescence and DAPI staining are on the left and right, respectively. Cells harbouring small cytoplasmic foci (arrows) and larger granules are illustrated in A and B, respectively. (C,D) Localization of CPEB1 after stable transfection. HeLa/CPEB1-lg cells were induced for 16 hours with doxycycline, fixed and stained with DAPI (C) or observed live (D). The arrows indicate small cytoplasmic foci. Bar, 8 µm.





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