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Fig. 9. Human p54 colocalizes with CPEB1 in both dcp1 and stress granules. (A) p54 is found in dcp1 bodies. HeLa cells were transiently transfected with an expression vector for RFP-tagged p54. After 20 hours, cells were fixed, stained with anti-dcp1 antibodies (green), and observed by confocal microscopy. (B) p54 colocalizes with CPEB1. HeLa cells were cotransfected with RFP-tagged p54 and GFP-tagged CPEB1-long. After 20 hours, cells were fixed and observed by confocal microscopy. Cells without (upper panel) and with stress granules (lower panel) are presented. (C,D) p54 is recruited with endogenous CPEB1 in stress granules induced with arsenite. HeLa cells were transiently transfected with an expression vector for RFP-tagged p54. After 20 hours, cells were stressed with arsenite for 30 minutes, incubated in the absence of arsenite for 1 hour and fixed. After staining with anti-eIF3 (C) or anti-CPEB1 antibodies (D) (green), cells were observed by confocal microscopy. In C, two p54 foci contacting stress granules have been enlarged for better visualization.
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