|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
| ||||||||||||||||||||
Files in this Data Supplement:
Movie 1. Polarization and directional migration of CHO cells in a DC electric field of 300 mV/mm. Time-lapse video corresponding to Fig. 1A-C shows that CHO cells polarize and migrate directionally towards the cathode. The recording time is 3 hours with a frame interval of 10 minutes.
Movie 2. Golgi polarization lags behind membrane protrusion, but significantly enhances directional migration when it polarizes in the field direction. Bright field video (upper left panel) and fluorescence video (upper right panel) show cell movement and Golgi polarization corresponding to Fig. 6. Lower left panel is the merged video. The cell on the left was polarized downward before the onset of EF. Active membrane protrusion occurred at the cathodal side 30 minutes after the onset of the EF. Only the protrusion next to the Golgi persisted. Then the cell moved and polarized at the same time as the Golgi polarized towards the cathode. After this, net migration towards the cathode significantly increased. Note that the cell on the right-hand side did not move and the Golgi apparatus did not repolarize.
Movie 3. An electric field of 300 mV/mm predominates over other directional cues to direct cell migration in a monolayer wound model. Time-lapse videos corresponding to Fig. 8B (upper left), Fig. 8C (upper right), Fig. 8E (lower left) Fig. 8F (lower right). An EF of 300 mV/mm predominates over other directional cues to direct cell migration in the monolayer wound model (upper right), where cells moved away from the wound. BFA completely abolished Golgi polarization (Fig. 7D-F), but EF-directed migration persisted albeit at a significantly decreased level (lower two panels). Electric field direction and cell tracing are indicated in Fig. 8. The time-lapse videos were captured over 3 hours.
| ||||||||||||||||||||
