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Fig. 2. Cathodal polarization of Golgi requires actin polymerization and signalling of Src and PI 3-kinases. (A,C,D) EF-induced Golgi polarization was abrogated by incubation of the cells with Brefeldin A (BFA), Cytochalasin D (CyD), Wortmannin (WM, PI 3-kinase inhibitor) and PP2 (Src inhibitor). Toxin B, LS203 (cdc42 inhibitor) and Y27632 (ROCK inhibitor) decreased Golgi polarization significantly (P<0.0001), but not completely (P<0.0001) (A). (B) The percentage of cells with their morphology polarized towards the cathode was also scored. For drug treatment, CHO cells were pretreated with PP2 (20 µM), BFA (1 µM, 5 µM), CyD (0.2 µM), Y27632 (50 µM) or LS203 (100 ng/ml) for 2 hours, then subjected to an EF of 300 mV/mm for 3 hours, fixed and triple-stained with phalloidin (green), GM130 (red) and DAPI (blue). Golgi polarization was scored as in Fig. 1G. More than 200 cells from three independent experiments were scored for each condition. Bar, 10 µm.
