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Fig. 7. An electric field of 300 mV/mm predominates over other directional cues in directing Golgi polarization in the scratch-wound model. Monolayer culture of CHO cells were wounded and allowed to heal for 4 hours (A) without an EF; (B) with an EF applied in the normal healing direction, or (C) opposite to the normal healing direction (the EF was applied 1 hour after wounding for 3 hours). (C) An EF applied against the default healing direction polarized the Golgi away from the wound, completely ignoring the wound direction. Some degree of F-actin polarization in EF direction can be seen (C, arrowheads). The three cells on the right in C have been focally enhanced to show clearer cell contours. BFA completely inhibited both scratch wounding and EF-induced Golgi polarization (D-G). The cells were triple-labelled with F-actin (green), GM130 (red) and DAPI (blue). Cells along the wound edge were scored as polarized if the Golgi was orientated to the right (for A,B,D,E) or to the left (for C,F) as in Fig. 1G. The percentage of polarized cells±s.e.m. was calculated from the means of three independent experiments from 255595 cells (G). Bar, 10 µm.

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