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Fig. 3. LEF-1 and PITX2 synergistically activate the LEF-1 promoter. CHO cells were transfected as in Fig. 1, with CMV-PITX2A or CMV-LEF-1, or both, and the CMV empty expression vector. The activities are shown as mean fold activation compared with the LEF-1 promoter plasmid without PITX2 expression and normalized to ß-galactosidase activity (± s.e.m. from four independent experiments). LEF-1 expression did not change the levels of ß-galactosidase activity in the transfected cells.





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