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Fig. 6. The LEF-1 WRE represses PITX2 activation. (A) The WRE deletion in the LEF-1 promoter compared with the full-length LEF-1 promoter. (B) CHO cells were transfected as in Fig. 1, with CMV-PITX2 isoforms and the CMV empty expression vector with the appropriate LEF-1 luciferase reporter constructs. The activities are shown as mean fold activation compared with the LEF-1 promoter plasmid without PITX2 expression and normalized to ß-galactosidase activity (± s.e.m. from four independent experiments).





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