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Fig. 6. Sec5 protein is mislocalized in sec6^Ex15 germ-line clones. (A) Control egg chamber, stages 3 and 4, labeled with anti-Sec5 antibody (green) and Hoechst 33342 (blue). Sec5 concentrates on membranes and is enriched at the boundary between the oocyte and posterior follicle cells (arrow). (B) In sec6^Ex15 germ lines, labeled as in A, Sec5 protein is found in puncta (arrow), many of which are clustered towards the center of the egg chamber. (C,D) Sec5 localization (green) is compared with the submembranous actin cytoskeleton (gray) in sec6^Ex15 germ lines. (E1) GFP^– sec6^Ex15 germ lines (generated as in Fig. 5G) stained for Sec5 (red) and Hoechst 33342 (blue). GFP is shown in green. (E2) GFP^– sec6^Ex15 germ lines stained for Sec5 (green) and actin (red). (B-E) Sec5 accumulates in intracellular puncta (arrows) and any germ-line membranes separating nuclei have a patchy, discontinuous distribution of Sec5. The Sec5 puncta often accumulate near the clump of ring canals. All scale bars are 20 µm.

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