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Fig. 7. Effects of S1P and of chemical treatments on membrane current and conductance. Voltage-clamp step pulses between –80 and 0 mV in 10 mV steps from a holding potential of –60 mV were applied to the cells for 100 milliseconds. Representative current traces in control (A) and after S1P (B), GdCl3 (C), S1P + GdCl3 (D), DHCB (E) and S1P + DHCB (F) treatments. G, normalized Im versus Vm plots in Control condition (
) and after S1P (
) or GdCl3 (
) or S1P + GdCl3 (
) or DHCB(
) or S1P + DHCB(
) treatment. The best linear regression lines are superimposed on control (continuous line) and the other experimental conditions (dashed lines). The calculated Cm, Gm and Gm/Cm values for all experimental conditions are reported in Table 1. Data are the mean±s.e.m.
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