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Fig. 9. Ca²⁺ influx after the application of stretching forces. Native C2C12 cells were pre-loaded with Fluo-3 (1 µM) and mechanically stimulated using the cantilever (white line) of an AFM. Arrow points to the cell to be stretched. The images were recorded by a cooled CCD camera prior and after the cantilever had been lifted off the cell at the indicated time points. The myoblast responds to the mechanical stimulus by a rise in intracellular Ca²⁺ that propagates from the stretched cell to the adjacent one. The images are representative of at least three independent experiments with similar results.

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