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Fig. 9. The ERK1/2 pathway is also required for MMP-1 overexpression following Cdc42 silencing with a second siRNA. (A) Representative western-blot analysis of whole-cell lysates of starved HSFs 72 hours after transfection with calcium phosphate alone (CaP) or with the indicated concentrations of an irrelevant siRNA (siScr) or the second siRNA targeting Cdc42 (2nd siCdc42). Cell lysates were analysed by immunoblotting with specific antibodies to phospho-ERK1/2 and ERK1/2. (B,C) Representative western-blot analysis of serum-free medium conditioned by HSFs for 16 hours between days 2 and 3 after transfection with 20 nM irrelevant siRNA (siScr), with 20 nM first siRNA targeting Cdc42 (1st siCdc42) or with 20 nM second siRNA targeting Cdc42 (2nd siCdc42) and cultured between days 1 and 3 after transfection with the indicated concentrations of the MEK-kinase inhibitor U0126 (B) or the p38-MAP-kinase inhibitor SB203580 (C).
