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Fig. 7. {alpha}3ß1 promotes stability of MMP-9 mRNA. MK+/+ cells, MK–/– cells, or MK–/– cells transfected with {alpha}3 grown on LN-5-ECM were pre-treated with cycloheximide to promote accumulation of MMP-9 mRNA in MK–/– cells, and then grown under serum-free conditions in the presence of actinomycin D to inhibit new transcription (see text for details). Total RNA was isolated at various time points and RT-PCR was performed to monitor turnover of mRNA for MMP-9 (A) and ß-actin (B). Results are plotted as the percentage of mRNA remaining relative to the starting amounts at 0 hour; diamonds, MK+/+ cells; squares, MK–/– cells; open circles, {alpha}3-transfected MK–/– cells. Data are presented as the mean±s.e.m. for three separate experiments; *P<0.05, one-way ANOVA with Newman Keuls post-hoc test. Gels show results from a representative experiment.





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