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Fig. 4. E-like targets tubulin for degradation via the proteosome pathway. (A) 293T cells were transfected with E-like-EGFP (El-EGFP) or EGFP alone (EGFP). Twenty-four hours following transfection, cells were incubated either in the presence (+) or absence (–) of the proteosome inhibitor MG132 (P.I.). Cell lysates were analyzed by SDS-PAGE and by western blotting using a 
-tubulin antibody for the detection of endogenous tubulin dimers and a ß-actin antibody as a loading control. (B) HeLa cells were transfected with the identical constructs described in A. Arrows indicate tubulin staining in cells overexpressing E-like. Fixed cells were examined by indirect immunofluorescence for the detection of tubulin and microtubules using a Texas Red-conjugated anti--tubulin antibody. Bar, 10 µm.
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