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Fig. 7. Reorganization of membranes in E-like depleted cells. (A) Clustering of endocellular membranes upon E-like depletion. Control (Sc) and E-like (El) knocked down cells were stained with antibodies against calnexin, giantin or lamp-2 for the detection of the ER, the Golgi complex and lysosomal membranes, respectively. (B) The ER clusters at the MTOC in E-like depleted cells (arrow). E-like silenced cells were immunolabeled with anti-calnexin and anti-acetylated tubulin for the detection of the ER and the MTOC, respectively. Bar, 10 µm.