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Fig. 6. Antibodies against Bet3 functionally inhibit the cytosolic pool of Bet3. Semi-intact NRK cells (lanes 4, 7, 10) were incubated with 2 µg of the indicated antibodies for 1 hour on ice, washed by centrifugation and resuspended in standard transport cocktail. Likewise, cytosol (lanes 5, 8, 11) or combinations of semi-intact cells and cytosol (lanes 3, 6, 9) were incubated with 2 µg of the indicated antibodies for 1 hour on ice. After supplementing with complete transport cocktail, assays were then incubated for 60 minutes on ice (lane 1) or at 32°C (lanes 2 to 11). The extent of VSV-G transport was monitored (A) and quantified (B).





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