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Fig. 7. Transfection with {alpha}5 integrin plasmid rescues osteoblast attachment on fibronectin in FGFR2 mutant osteoblasts. (A) FGFR2 mutant osteoblasts were transfected with {alpha}5 plasmid (pcDNA-{alpha}5) or the empty vector (pcDNA). After 24 hours, cell lysates were immunoprecipitated (IP) with anti-{alpha}5 integrin, resolved by SDS-PAGE and blotted with {alpha}5 integrin antibody. (B) FGFR2 mutant osteoblasts transfected with {alpha}5 integrin plasmid or empty vector were plated on fibronectin-coated dishes, cultured for 48 hours and the number of adherent cells was counted. Data are the mean±s.e.m. of six wells; a and b indicate a significant difference with control and pcDNA-mutant cells, respectively (P<0.05).





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