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Fig. 8. Restoration of {alpha}5 integrin levels abolishes caspase-dependent apoptosis induced by FGFR2 activation in osteoblasts. FGFR2 mutant osteoblasts were transfected with the empty vector (pcDNA) or the {alpha}5 integrin plasmid (pcDNA-{alpha}5) and cultured for 48 hours in medium with 1% FCS. Cell lysates were immunoprecipitated (IP) with Bax or Bcl-2 antibodies, resolved by SDS-PAGE and blotted with anti-Bax (A) or anti-Bcl-2 (B). Caspase-9 activity (C) and caspase-3 activity (D) were determined in FGFR2 mutant and control osteoblasts and the levels were corrected for protein content. Data are the mean±s.e.m. of three wells; a and b indicate a significant difference with control and pcDNA-mutant cells, respectively (P<0.05).





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