QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 5. Wild-type T-plastin stabilizes actin filaments in a Ca²⁺-independent manner. (A) Depolymerization of T-plastin-decorated pyrene-labeled F-actin in the presence of increasing free Ca²⁺ concentrations. G-Actin (4 µM, 25% pyrene labeled) was co-polymerized with T-plastin (4 µM) in the presence of increasing concentrations of free Ca²⁺ (4.6 nM to 1.6 µM). F-Actin was diluted and depolymerization was measured as described in Fig. 4. Control (actin) was the depolymerization of F-actin in the absence of T-plastin. (B) Cosedimentation of T-plastin with F-actin in the presence of increasing free Ca²⁺ concentrations. G-Actin (7 µM) was co-polymerized with T-plastin (3 µM) in the presence of increasing concentrations of free Ca²⁺. Proteins were centrifuged at high speed to sediment actin filaments. Proteins in supernatants (S) and pellets (P) were separated by SDS-PAGE under reducing conditions. Protein bands were stained with Coomassie Brilliant Blue. The migration positions of plastin and actin are indicated. Notice that, owing to the presence of Ca²⁺, more actin is present in the supernatant than observed in the experiment in Fig. 2.

Return to article