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Fig. 7. ABD1 stabilizes F-actin structures in transfected cells. (A) ABD1 induces the formation of long actin cables in cells to which the actin-binding proteins {alpha}-actinin and VASP are recruited. Transfected Vero cells transiently expressing epitope-tagged ABD1-vsv were processed for triple staining with Alexa-320/phalloidin as a probe for F-actin (left), anti-vsv tag primary antibody and Cy2-coupled secondary antibody for immunofluorescence staining of ABD1-vsv (middle), anti-{alpha}-actinin antibody and Texas-Red-coupled secondary antibody (right, top) or anti-VASP antibody and Texas-Red-coupled secondary antibody (right, bottom). Bar, 10 µm. Notice that cells expressing ABD1 exhibit much-increased F-actin staining and many actin cables, which are not detected in neighboring untransfected cells. (B) F-Actin cables induced by ABD1 are more resistant to latrunculin-A treatment than those of non-transfected cells. Transfected Vero cells were treated with 100 nM latrunculin A for 60 minutes and double stained for F-actin (left) and vsv-tag (right). Bar, 15 µm. Notice that the actin cytoskeleton of untransfected cells (arrows) is disrupted compared with that of cells expressing ABD1.





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