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Fig. 1. Brain-specific expression of Necl-1. (A) Specific recognition of Necl-1 with anti-Necl-1 pAb. Cell lysates (10 µg of protein each) of Necl-1-L, Necl-2-L and wild-type L cells were subjected to SDS-PAGE (10%), followed by western blotting with anti-Necl-1 pAb. (B) Tissue distribution of Necl-1. Homogenates of various mouse tissues (20 µg of protein each) were subjected to SDS-PAGE (10%), followed by western blotting with anti-Necl-1 pAb. (C) Distribution of Necl-1 in the brain. Homogenates of various regions of rat brain (20 µg of protein each) were subjected to SDS-PAGE (10%), followed by western blotting with anti-Necl-1 pAb. (D) Developmental expression of Necl-1 in the brain. Homogenates of various developmental stages of total rat brain (20 µg of protein each) were subjected to SDS-PAGE (10%), followed by western blotting with anti-Necl-1 pAb. The results shown are representative of three independent experiments.
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