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Fig. 2. Localization of Necl-1 in the molecular layer of the cerebellum. (A,B) Immunofluorescence images of mouse cerebellum. Frozen sections of mouse cerebellum were double-stained with anti-Necl-1 pAb and anti-synaptophysin mAb. Aa and Ba, Necl-1 (red); Ab and Bb, synaptophysin (green); Ac and Bc, merge. ML, molecular layer; P, Purkinje cells; GL, granular layer. Bars, 10 µm. The results shown are representative of three independent experiments.





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