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Fig. 3. Localization of Necl-1 at non-junctional cell-cell contact sites in the molecular layer of the cerebellum. Samples were stained with anti-Necl-1 pAb followed by immunogold electron microscopy with the silver-enhanced immunogold method. (A,B) The molecular layer in mouse cerebellum. Open arrowheads, synaptic junctions; red arrows, contact sites between a parallel fiber terminal or axon and a glia cell; green arrows, contact sites of two parallel fiber terminals. (A',B') Schematic drawings of A and B. Pre, parallel fiber terminals or axons of granular cells; Post, Purkinje cell dendrite synapses; Glia, glia cell processes. Bars, 250 nm. The results shown are representative of three independent experiments.





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