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Fig. 6. Dyn3baa-induced filopodial outgrowth in mature neurons requires both the PRD of Dyn3 and full-length cortactin. (A) Dyn3aaa and Dyn3baa were truncated by 115 amino acids to remove the PRD and putative cortactin binding site. (B) Dyn3aaa{Delta}PRD does not affect synaptogenesis. Neurons transfected with Dyn3aaa{Delta}PRD-GFP show normal spine morphology at 18DIV. (C) Dyn3baa{Delta}PRD does not induce filopodial outgrowth in mature neurons. Neurons transfected with Dyn3baa{Delta}PRD-GFP exhibit normal spine morphology at 18DIV. (D) Cortactin was truncated by 73 amino acids, removing the SH3 domain and putative dynamin binding site. (E) Neurons co-transfected with Dyn3aaa and Cort{Delta}SH3-RFP display no morphological defects in mature spine development. (F) Dyn3baa requires full-length cortactin to cause filopodial outgrowth in mature neurons. Neurons co-transfected with Dyn3baa-GFP and Cort{Delta}SH3-RFP exhibit normal spine morphology at 18DIV, unlike neurons singly transfected with Dyn3baa-GFP. Scale bar, 5 µm.





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