spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 3. (A) Analysis of p25-Cdk5 complex. Cell lysates from NGF-p25 and NGF-mock cells (used as negative control), treated for 6 hours with tetracycline, were subjected to immunoprecipitation (IP) by antibodies against either the p35 C-terminus or Cdk5. Immune complexes were then analysed by western blotting (WB) with antibodies against the p35 C-terminus and Cdk5. (B) Analysis of roscovitine's effect on earlier Rb phosphorylation. NGF-differentiated p25 cells were induced (+) or not (–) with tetracycline for 6 hours in the absence or the presence of 5 µM roscovitine (–/+). Lysates were immunoblotted with antibody against phospho-Ser807/811 Rb (P-Rb) followed, after stripping, with the phosphorylation-independent antibody (IF-8) against Rb protein (Rb).





Right arrow Return to article