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Fig. 5. Colocalization of lipoprotein receptors with Tf in the ERC. Incubation of CHO(LpR) (A), CHO(LpR_1-790LDLR_791-839) (B), ldlA(LpR_1-342LDLR_293-839) (C), ldlA(LDLR) (D), ldlA(LDLR_1-292LpR_343-850) (E), ldlA(LDLR_H562N) (F) and ldlA(LDLR_H562Y) transfectants (G) for 15 minutes with OG-Tf followed by a short chase of 10 minutes in growth medium shows that Tf accumulates in the ERC (left, grey). IF using anti-LpR antibody 2189/90 (A-C) or anti-LDLR antibody C7 (D-G) reveals that all receptors (middle, grey), except LpR_1-342LDLR_293-839 (C), also converge in this organelle and colocalize with Tf (right – Tf, green; receptor, red; colocalization, yellow). Scale bar, 10 µm (G, right).

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