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Fig. 2. Large TIN2 domains are often perinucleolar and colocalized with dense HP 1{gamma} foci. (A) Dual immunostaining for TIN2 (green) and nucleolin (red) in S1 acini in 3D culture. The image is a confocal section of an acinus containing several nuclei, illustrated by the drawing on the left. (B) Dual staining for TIN2 (green) and PML (red) in S1 acini. 3D cultures were untreated (control) or treated for 30 minutes with DNase I (DNase) or RNase A (RNase) prior to immunostaining. (C) Dual immunostaining for HP 1{gamma} (red) and TIN2 (green) in S1 acini. Arrows indicate HP 1{gamma} domains that overlap with large TIN2 domains, arrowheads indicate large TIN2 domains and dashed lines delineate the nuclear periphery. Bar, 5 µm.





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