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Fig. 8. HBZ localization in heterochromatin of transfected COS cells. COS cells transfected with pcDNA3.1(–)/Myc-His-HBZ (A-D) or pcDNA3.1(–)/Myc-His (E-F) for 36 hours were fixed with 2.5% formaldehyde and embedded in Lowicryl resin. Ultrathin sections were stained with the mouse anti-Myc antibody, then incubated with goat anti-mouse IgG antibody conjugated to 10 nm gold particles, and analyzed by electron microscopy. Bar, 0.5 µm. NU, nucleus; NO, nucleolus.





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