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Fig. 10. Visualization of T7-Syt I and Syt II glycosylation mutants. RBL cells transiently transfected with T7-Syt I(T26/A) (a) or T7-Syt I(T15/16/A) (b) or Syt II(T17/18/19/A) (c) were grown on glass coverslips for 24 hours and subsequently labeled with anti-T7 monoclonal antibodies (a,b) or polyclonal antibodies directed against C2A-Syt IX (c) followed by the relevant Cy3-conjugated (a,c) or Cy2-conjugated secondary antibodies. Cells were processed for immunofluorescent staining and visualized by confocal microscopy. The z-line image is of the same field as in b and confirms the plasma membrane localization of the {Delta}O mutant. Bar, 10 µm.





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