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Fig. 4. Subcellular fractionation of HA-Syt I/Syt II- or HA-Syt II/Syt I-expressing RBL cells. Cell homogenates derived from RBL cells transiently transfected with HA-Syt I/Syt II or HA-Syt II(1-183aa)/Syt I cDNA were fractionated on continuous sucrose gradients as described previously (Baram et al., 1999). Fractions were collected from the top, subjected to SDS-PAGE and immunoblotted with anti-HA antibodies. Fractions were also examined for ß-hexosaminidase activity (presented as OD at 405 nm).





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