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Fig. 8. Western blot analysis of HA-Syt II expressed in RBL cells. Cell extracts (80 µg) derived from RBL cells stably transfected with either (A) non-tagged Syt II (1) or HA-Syt II (2) cDNA, or (B) Syt II-GFP or Flag-Syt II-GFP cDNA or (C) transiently transfected with T7-Syt I, T7-Syt I(T15/16/A) or T7-Syt I(T26/A) cDNA, were resolved by SDS-PAGE and subjected to immunoblotting with either monoclonal anti-Syt II or anti-HA antibodies as indicated (A), or anti-GFP antibodies (B), or anti-T7 antibodies (C). The positions of the molecular mass markers are shown on the right in A. Arrows point to fully, partially or non-modified immunoreactive proteins.





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