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Fig. 6. 4E-BP and the 40 kDa proteins re-appear during late embryogenesis of sea urchin. (A) Total extracts of unfertilized eggs or eggs at the indicated times following fertilization were subjected to western blotting using 4E-BP2 or eIF4E antibodies. Tubulin antibody was used as a loading control. Data are representative of at least five independent experiments. (B) Total extracts prior to fertilization or at the indicated times following fertilization were subjected to far-western blotting using an eIF4E-HMK probe. Data are representative of two independent experiments. (C) Proteins affinity purified using an m7GTP column prior to fertilization or at the indicated times following fertilization were subjected to western blotting using 4E-BP or anti-eIF4E antibodies. Data are representative of at least two independent experiments.





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